EDN2 is a member of the endothelin protein family of secretory vasoconstrictive peptides. The preproprotein is processed to a short mature form which functions as a ligand for the endothelin receptors that initiate intracellular signaling events. This protein is involved in a wide range of biological processes, such as hypertension and ovulation. Recombinant human EDN2 protein, fused to His-tag at N-terminus, was expressed in E.coli.