EAF1 belongs to the EAF family and is strongly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine and colon. This protein acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Recombinant human EAF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.