DDX56 is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX56 shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. Recombinant human DDX56 protein, fused to His-tag at N-terminus, was expressed in E.coli.