Cytosolic non-specific dipeptidase, also known as CNDP2, is a cytosolic, non-specific dipeptidase that belongs to the peptidase M20A family of proteins. CNDP2 is a secreted peptidase homologous to M20 peptidases. It is expressed by all adult and fetal tissue, however, an isoform lacking exons 3 and 4 was expressed in all fetal tissue, but only in adult liver. Overexpression of CPGL-B in hepatocellular carcinoma cells leads to significant inhibition of HC cell viability, colony formation, cell invasiveness and tumor formation. Recombinant human CNDP2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.