CLU, also known as clusterin, is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants. Recombinant human CLU protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.