Calcium/calmodulin-dependent protein kinase II, also known as CAMK2N2, is potent and specific cellular inhibitor of CaM-kinase II (CAMK2). This protein may play an important role in the regulation of cell growth when overexpressed in colon adenocarcinoma LoVo cells. Recombinant human CAMK2N2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.