BOLA3 also known as BolA-like protein 3 isoform1. BOLA3 is novel non-classical secreted protein and the secretion was not dependent on its predicted signal peptide. Human BolAs belongs to three different groups with functional divergence of BolA subfamily, where the different helix-turn-helix motif among BOLA 1, BOLA 2, and BOLA3 could be responsible for their functional divergence. Recombinant human BOLA3 was expressed in E.coli and purified by using conventional chromatography techniques.