ATP synthase subunit gamma, mitochondrial isoform L (liver), also known as ATP5C1, catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. Recombinant human ATP5C1 protein, fused to His-tag at N-terminus, was expressed in E.coli.