ANTXR2, also known as anthrax toxin receptor 2, is a widely expressed anthrax toxin receptor family protein. The main functional domain of this protein is an extracellular integrinlike von Willebrand factor type A (VWA) domain with a metal ion dependent adhesion site, through which it adheres selectively to collagen type IV and laminin. This protein and the related protein ATR/TEM8 serve as receptors for the protective antigen of Bacillus anthracis. Recombinant mouse ANTXR2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.