ACSF2 also known as Acyl-CoA synthetase family member 2. Acyl-CoA synthetases are a family of enzymes that catalyze the thioesterification of fatty acids with coenzymeA to form activated intermediates, which play a fundamental role in lipid metabolism and homeostasis of lipid-related processes. ACSF2 are required for complex lipid synthesis, energy production via beta-oxidation, protein acylation and fatty-acid dependent transcriptional regulation. ACSF2 are also necessary for fatty acid import into cells by the process of vectorial acylation, Recombinant human ACSF2, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.