SMAC/Diablo, 56-239aa, Human, T7 tagged, Recombinant, E.coli

Smac/Diablo is one of the proapoptotic proteins. Smac promotes caspase activation in the cytochrome c/Apaf-1/caspase-9 pathway by binding to inhibitor of apoptosis proteins (IAPs) and removing their inhibitory activity. Recombinant human Smac fused to T7-tag. at N-terminus was expressed in E.coli and purified by conventional chromatography techniques.

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Properties

Data Sheet	Click for Datasheet
Catalog Number	TP03964
Size	100 µg
Host	E.coli
Accession
Molecular Weight	22 kDa (199 aa), confirmed by MALDI-TOF.
AP_Mol_Weight
Tag
Sequences	MASMTGGQQMGRGSMAVPIAQKSEPHSLSSEALMRRAVSLVTDSTSTFLSQTTYALIEAITEYTKAVYTLTSLYRQYTSLLGKMNSEEEDEVWQVIIGARAEMTSKHQEYLKLETTWMTAVGLSEMAAEAAYQTGADQASITARNHIQLVKLQVEEVHQLSRKAETKLAEAQIEELRQKTQEEGEERAESEQEAYLRED
Purity	> 95% by HPLC
Concentration	1 mg/ml (determined by Bradford assay)
Formulation	Liquid in 20mM Tris pH 7.5
Other Names	SMAC3, DIABLO-S, FLJ10537, FLJ25049, Diablo isoform 1, SMAC/Diablo, Diablo isoform 1 0610041G12Rik, DBOH, Diablo homolog, DIABLO S, Diablo homolog (Drosophila), Mitochondrial Smac protein, Diablo homolog mitochondrial, SMAC 3, Smac protein.
Bioactivity
Storage	Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Postscript	For research use only, not for use in diagnostic procedures.

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SMAC/Diablo, 56-239aa, Human, T7 tagged, Recombinant, E.coli

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