RGMa Anti-Rat Rabbit IgG Affinity Purify

RGMa (Repulsive Guidance Molecule a) is a GIP-anchored protein, about 33 kDa, and it is implicated in axonal guidance and neural tube closure of embryonic retina (ref. 1, 2, 3). It has reported as a potent inhibitor of axonal regeneration in the adult central nervous system (CNS). It is speculated that RGMa inhibits neurite outgrowth by activating the RhoA-Rho kinase signaling pathway in vitro. While, it has reported that, in a rat SCI (spinal cord injury) model, neurite outgrowth is enhanced by administration of anti-RGMa antibody to lesion site, resulting they are improved in mortor functional recovery (ref. 4). Thus function analysis of RGMa is attracting attention in a research field of axonal regeneration of CNS. This antibody detects rat RGMa and can be used for western blotting, immuno-precipitation and FACS analysis. And it is also reported that administration of the antibody to cerebellar granule cells of 6 - 8 days-old rat, in vitro, neutralizes RGMa-dependent inhibition of neurite outgrowth (ref. 4).