POLR1C, 1-349aa, Human, His tag, E.coli

POLR1C is a subunit of both RNA polymerase I and RNA polymerase III complexes. The encoded protein is part of the Pol core element. Defects in this gene have been associated with Treacher Collins syndrome (TCS). Recombinant human POLR1C protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Properties

Data Sheet	Click for Datasheet
Catalog Number	TP03457
Size	50 µg
Host	E.coli
Accession
Molecular Weight	41.6 kDa (369aa) confirmed by MALDI-TOF
AP_Mol_Weight
Tag	N-6His
Sequences	MGSSHHHHHHSSGLVPRGSHMGSMAASQAVEEMRSRVVLGEFGVRNVHTTDFPGNYSGYDDAWDQDRFEKNFRVDVVHMDENSLEFDMVGIDAAIANAFRRILLAEVPTMAVEKVLVYNNTSIVQDEILAHRLGLIPIHADPRLFEYRNQGDEEGTEIDTLQFRLQVRCTRNPHAAKDSSDPNELYVNHKVYTRHMTWIPLGNQADLFPEGTIRPVHDDILIAQLRPGQEIDLLMHCVKGIGKDHAKFSPVATASYRLLPDITLLEPVEGEAAEELSRCFSPGVIEVQEVQGKKVARVANPRLDTFSREIFRNEKLKKVVRLARVRDHYIFSVESTGVLPPDVLVSEAIKVLMGKCRRFLDELDAVQMD
Purity	> 95% by HPLC
Concentration	0.5 mg/ml (determined by Bradford assay)
Formulation	Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Other Names	DNA-directed RNA polymerases I and III subunit RPAC1, RP3-337H4.4, RPA39, RPA40, RPA5, RPAC1, TCS3
Bioactivity
Storage	Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Postscript	For research use only, not for use in diagnostic procedures.

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POLR1C, 1-349aa, Human, His tag, E.coli

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