POGLUT1, 24-392aa, Human, His tag, Baculovirus

Categories: [Proteins / Peptides]
POGLUT1, also known as protein O-glucosyltransferase 1, is a homologue of Rumi from Drosophila, an endoplasmic reticulum (ER)-retaining glucosyltransferase that adds glucose to serine residues within the consensus sequence of C1-X-S-X-P-C2 in Notch EGF repeats, thereby regulating cell-fate decisions. Recombinant human POGLUT1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
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Properties

Data Sheet Click for Datasheet
Catalog Number TP03452
Size 20 µg
Host Baculovirus
Accession
Molecular Weight 44.5kDa (377aa); 40-57KDa (SDS-PAGE under reducing conditions)
AP_Mol_Weight
Tag N-6His
Sequences RQKESGSKWKVFIDQINRSLENYEPCSSQNCSCYHGVIEEDLTPFRGGISRKMMAEVVRRKLGTHYQITKNRLYRENDCMFPSRCSGVEHFILEVIGRLPDMEMVINVRDYPQVPKWMEPAIPVFSFSKTSEYHDIMYPAWTFWEGGPAVWPIYPTGLGRWDLFREDLVRSAAQWPWKKKNSTAYFRGSRTSPERDPLILLSRKNPKLVDAEYTKNQAWKSMKDTLGKPAAKDVHLVDHCKYKYLFNFRGVAASFRFKHLFLCGSLVFHVGDEWLEFFYPQLKPWVHYIPVKTDLSNVQELLQFVKANDDVAQEIAERGSQFIRNHLQMDDITCYWENLLSEYSKFLSYNVTRRKGYDQIIPKMLKTELLEHHHHHH
Purity > 95% by HPLC
Concentration 0.25mg/ml (determined by Absorbance at 280nm)
Formulation Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Other Names Protein O-glucosyltransferase 1, POGLUT1, C3orf9, CLP46, hCLP46, KDELCL1, KTELC1
Bioactivity
Storage Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Postscript For research use only, not for use in diagnostic procedures.

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