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Osteopontin, 17-314aa, Human, His-tagged, Recombinant, E.coli

Categories: [Proteins / Peptides]
Osteopontin (OPN), also known as Secreted phosphoprotein 1 (SPP1), is a human gene product which is conserved in other species. This protein is glycoprotein that was first identified in osteoblasts and plays an important role in bone remodeling, immune functions in fibroblasts, macrophages, and lymphocytes during inflammation and wound healing.
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Properties

Data Sheet Click for Datasheet
Catalog Number TP03264
Size 100 µg
Host E.coli
Accession
Molecular Weight 36.2 kDa (321aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will shift up)
AP_Mol_Weight
Tag
Sequences MGSSHHHHHHSSGLVPRGSHRSMIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSNESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRISHELDSASSEVN
Purity > 95% by HPLC
Concentration 1 mg/ml (determined by Bradford assay )
Formulation Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT,10% glycerol, 2 mM EDTA
Other Names OPN, SPP-1, BNSP, BSPI, ETA-1
Bioactivity
Storage Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Postscript For research use only, not for use in diagnostic procedures.
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