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OCT4, 1-265aa Human, His tag, E.coli

Categories: [Proteins / Peptides]
POU domain, class 5, transcription factor 1, also known as OCT4, binds to the octamer motif (5'-ATTTGCAT-3'). This protein forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. It is critical for early embryogenesis and for embryonic stem cell pluripotency. Recombinant human OCT4 protein, fused to His-tag at N-terminus, was expressed in E.coli.
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Properties

Data Sheet Click for Datasheet
Catalog Number TP03244
Size 100 µg
Host E.coli
Accession
Molecular Weight 32.2 kDa (285aa)
AP_Mol_Weight
Tag N-6His
Sequences
Purity > 95% by HPLC
Concentration 0.5 mg/ml (determined by Bradford assay)
Formulation Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Other Names POU domain, class 5, transcription factor 1 isoform 2, POU5F1, MGC22487, OCT3, OCT4, OTF3, OTF4
Bioactivity
Storage Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Postscript For research use only, not for use in diagnostic procedures.
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