Radioimmunoassay (RIA) for the determination Neuropeptide Y (NPY) in human serum and plasma. For research use only, not for use in diagnostic procedures.
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Properties

Protocol PDF Click for Protocol
Catalog Number TK01538
Species Human
Design Radioimmunoassay (RIA) technique.
Standards 7 standards, serially diluted from 1 prepared lyophilized standard.
Controls 2 controls, lyophilized.
Sample Types Human serum or plasma (EDTA, Heparin).
Sample Volume 200 µL / determination.
Assay Desc. 20 hour incubation (2-8°C) + 20 hours (2-8°C) + 30 min. (2-8°C) + Centrifuge 15 min. (4°C) = 40 hours, 45 min. total incubation time.
Standard Range 0 / 9.4 - 300 pmol/L.
Sensitivity 3 pmol/L
Radioactivity
Other Names For research use only, not for use in diagnostic procedures.
Storage 2 - 8°C
Postscript For research use only, not for use in diagnostic procedures.

Intended use

This ELISA kit applies to the in vitro quantitative determination of Neuropeptide Y concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antigen specific to Neuropeptide Y. Standards or samples are added to the micro ELISA plate wells and combined with the specific antigen. Then a biotinylated detection antigen specific for Neuropeptide Y and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Neuropeptide Y, biotinylated detection antigen and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Neuropeptide Y. You can calculate the concentration of Neuropeptide Y in the samples by comparing the OD of the samples to the standard curve.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Precision

Intra-assay Precision (Precision within an assay):
3 samples with low, middle,high concentration of Neuropeptide Y. And the sample of Neuropeptide Y were tested 30 times on one plate, respectively.
Inter-assay Precision (Precision between assays):
3 samples with low, middle,high concentration of Neuropeptide Y. And the sample of Neuropeptide Y were tested on 3 different plates, 30 replicates in each plate.

Recovery

Matrices listed below were spiked with certain level of Neuropeptide Y and the recovery rates were calculated by comparing the measured value to the expected amount of Neuropeptide Y in samples.

Matrix Recovery range (%) Average Recovery (%)
Serum (n=5) 92-105 97
EDTA plasma (n=5) 90-104 96
heparin plasma (n=5) 87-99 94

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neuropeptide Y and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

    Serum (n=5) EDTA plasma (n=5) heparin plasma (n=5)
1:2 Range (%) 93-102 92-108 87-102
Average (%) 93 94 93
1:4 Range (%) 87-100 90-101 94-99
Average (%) 94 95 95
1:8 Range (%) 93-99 94-99 92-104
Average (%) 94 94 94
1:16 Range (%) 91-108 94-99 92-102
Average (%) 95 95 94

Stability

The stability of Neuropeptide Y is determined by the loss rate of activity. The loss rate of Neuropeptide Y is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Item Specifications Storage
Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20℃, 6 months
Reference Standard 2 vials
Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 4℃, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 4℃(shading light)
Stop Solution 1 vial, 10 mL 4℃
Plate Sealer 5 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Other supplies required

Microplate reader with 450 nm wavelength filter
High-precision transfer pipette, EP tubes and disposable pipette tips
Incubator capable of maintaining 37℃
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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