NANOG, 1-154aa, Human, His tag, E.coli

NANOG, also known as nanog homeobox, is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is down-regulated, cell differentiation can proceed.

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Properties

Data Sheet	Click for Datasheet
Catalog Number	TP03087
Size	50 µg
Host	E.coli
Accession
Molecular Weight	19.6 kDa (174aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
AP_Mol_Weight
Tag	N-6His
Sequences	MGSSHHHHHHSSGLVPRGSHMSVDPACPQSLPCFEASDCKESSPMPVICGPEENYPSLQMSSAEMPHTETVSPLPSSMDLLIQDSPDSSTSPKGKQPTSAEKSVAKKEDKVPVKKQKTRTVFSSTQLCVLNDRFQRQKYLSLQQMQELSNILNLSYKQVKTWFQNQRMKSKRWQ
Purity	> 95% by HPLC
Concentration	0.25 mg/ml (determined by Bradford assay)
Formulation	Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT
Other Names	Homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48
Bioactivity
Storage	Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Postscript	For research use only, not for use in diagnostic procedures.

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NANOG, 1-154aa, Human, His tag, E.coli

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