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MPG, 1-298aa, Human, His tag, E.coli

Categories: [Proteins / Peptides]
MPG (DNA-3-methyladenine glycosylase) belongs to the DNA glycosylase MPG family. This protein initiates base excision repair in DNA by removing a wide variety of alkylated, deaminated, and lipid peroxidationinduced purine adducts. Recombinant human MPG protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
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Properties

Data Sheet Click for Datasheet
Catalog Number TP03010
Size 50 µg
Host E.coli
Accession
Molecular Weight 33.9kDa (306aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
AP_Mol_Weight
Tag N-6His
Sequences MVTPALQMKKPKQFCRRMGQKKQRPARAGQPHSSSDAAQAPAEQPHSSSDAAQAPCPRERCLGPPTTPGPYRSIYFSSPKGHLTRLGLEFFDQPAVPLARAFLGQVLVRRLPNGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYIIYGMYFCMNISSQGDGACVLLRALEPLEGLETMRQLRSTLRKGTASRVLKDRELCSGPSKLCQALAINKSFDQRDLAQDEAVWLERGPLEPSEPAVVAAARVGVGHAGEWARKPLRFYVRGSPWVSVVDRVAEQDTQALEHHHHHH
Purity > 95% by HPLC
Concentration 0.25mg/ml (determined by Bradford assay)
Formulation Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 200mM NaCl, 1mM EDTA
Other Names DNA-3-methyladenine glycosylase, AAG, ADPG, anpg, APNG, CRA36.1, MDG, Mid1, PIG11, PIG16
Bioactivity
Storage Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Postscript For research use only, not for use in diagnostic procedures.
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