Mif, 1-115aa , Mouse, His tag, E.coli

Categories: [Proteins / Peptides]
Mif also known as Macrophage migration inhibitory factor. MIF has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. Recombinant mouse Mif, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques
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Properties

Data Sheet Click for Datasheet
Catalog Number TP02975
Size 100 µg
Host E.coli
Accession
Molecular Weight 14.9kDa (138aa) confirmed by MALDI-TOF
AP_Mol_Weight
Tag N-6His
Sequences MGSSHHHHHHSSGLVPRGSHMGSMPMFIVNTNVPRASVPEGFLSELTQQLAQATGKPAQYIAVHVVPDQLMTFSGTNDPCALCSLHSIGKIGGAQNRNYSKLLCGLLSDRLHISPDRVYINYYDMNAANVGWNGSTFA
Purity > 95% by HPLC
Concentration 0.5mg/ml (determined by Bradford assay)
Formulation Liquid. In Phosphate buffered saline containing 10% glycerol, 1mM DTT
Other Names Macrophage migration inhibitory factor, GIF, Glif
Bioactivity
Storage Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Postscript For research use only, not for use in diagnostic procedures.

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