IL-18 Human, Recombinant, E.coli

Categories: [Proteins / Peptides]
Interleukin 18 (IL-18), previously known as interferon-gamma (IFN-)-inducing factor, is an activator of NK cells and a co-inducer of Th1 cytokines. IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. Recombinant human IL-18 protein was expressed in E.coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
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Properties

Data Sheet Click for Datasheet
Catalog Number TP02592
Size 100 µg
Host E.coli
Accession
Molecular Weight 18.3kDa (158aa), confirmed by MALDI-TOF
AP_Mol_Weight
Tag
Sequences MYFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED
Purity > 95% by HPLC
Concentration 1 mg/ml (determined by Bradford assay)
Formulation Liquid. In Phosphate buffer saline (pH 7.4)
Other Names IL-18, IFN-gamma-inducing factor (IGIF), IL-1 gamma, Iboctadekin, IL1F4
Bioactivity
Storage Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Postscript For research use only, not for use in diagnostic procedures.

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