HMGCS1, 1-520aa, Human, His tag, E.coli

HMGCS1 also known as hydroxymethylglutaryl-CoA synthase, cytoplasmic. This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase. Recombinant human HMGCS1 was expressed in E.coli and purified by using conventional chromatography techniques

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Properties

Data Sheet	Click for Datasheet
Catalog Number	TP02472
Size	20 µg
Host	E.coli
Accession
Molecular Weight	59.7kDa (543aa)
AP_Mol_Weight
Tag	N-6His
Sequences	MGSSHHHHHHSSGLVPRGSHMGSMPGSLPLNAEACWPKDVGIVALEIYFPSQYVDQAELEKYDGVDAGKYTIGLGQAKMGFCTDREDINSLCMTVVQNLMERNNLSYDCIGRLEVGTETIIDKSKSVKTNLMQLFEESGNTDIEGIDTTNACYGGTAAVFNAVNWIESSSWDGRYALVVAGDIAVYATGNARPTGGVGAVALLIGPNAPLIFERGLRGTHMQHAYDFYKPDMLSEYPIVDGKLSIQCYLSALDRCYSVYCKKIHAQWQKEGNDKDFTLNDFGFMIFHSPYCKLVQKSLARMLLNDFLNDQNRDKNSIYSGLEAFGDVKLEDTYFDRDVEKAFMKASSELFSQKTKASLLVSNQNGNMYTSSVYGSLASVLAQYSPQQLAGKRIGVFSYGSGLAATLYSLKVTQDATPGSALDKITASLCDLKSRLDSRTGVAPDVFAENMKLREDTHHLVNYIPQGSIDSLFEGTWYLVRVDEKHRRTYARRPTPNDDTLDEGVGLVHSNIATEHIPSPAKKVPRLPATAAEPEAAVISNGEH
Purity	> 95% by HPLC
Concentration	0.5mg/ml (determined by Bradford assay)
Formulation	Liquid. In Phosphate buffered saline (pH7.4) containing 10% glycerol, 1mM DTT
Other Names	Hydroxymethylglutaryl-CoA synthase, cytoplasmic, HMGCS
Bioactivity
Storage	Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Postscript	For research use only, not for use in diagnostic procedures.

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HMGCS1, 1-520aa, Human, His tag, E.coli

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