GroES, E.coli,Recombinant, expressed in E.coli

Categories: [Proteins / Peptides]
GroES protein is the co-chaperonin of GroES in E.coli and assists protein folding. GroEL mediated folding requires the co-chaperonin GroES which is essential for viability. GroES is composed of a single heptameric ring of 10kDa subunits that binds to the ends of the GroEL cylinder. GroES gene was amplified by PCR from E.coli and cloned into an expression vector. This protein was overexpressed in E.coli and was purified by using conventional chromatography techniques.
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Properties

Data Sheet Click for Datasheet
Catalog Number TP02335
Size 100 µg
Host E.coli
Accession
Molecular Weight 10.4kDa (97aa), confirmed by MALDI-TOF
AP_Mol_Weight
Tag
Sequences MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVGNGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA
Purity > 95% by HPLC
Concentration 1 mg/ml (determined by Bradford assay)
Formulation Liquid. In 25 mM Tris buffer (pH 7.5) containing 100 mM NaCl,5 mM DTT, 10% glycerol
Other Names 10 kDa chaperonin, Protein Cpn10, groS, mopB
Bioactivity
Storage Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Postscript For research use only, not for use in diagnostic procedures.

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