GLTPD1, 1-214aa, Human, His tag, E.coli

GLTPD1 mediates the intracellular transfer of ceramide-1-phosphate between organelle membranes and the cell membrane. This protein is required for normal structure of the Golgi stacks. GLTPD1 can bind phosphoceramides with a variety of aliphatic chains, but has a preference for lipids with saturated C16:0 or monounsaturated C18:1 aliphatic chains, and is inefficient with phosphoceramides containing lignoceryl (C24:0). It plays a role in the regulation of the cellular levels of ceramide-1-phosphate, and thereby contributes to the regulation of phospholipase PLA2G4A activity and the release of arachidonic acid. Recombinant human GLTPD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.