GADD45A, 1-165aa, Human, His tag, E.coli

GADD45A, also known as growth arrest and DNA damage-inducible protein, binds both Cdks and PCNA, a protein involved in DNA replication and repair. It has been shown to stimulate DNA excision repair in vitro and to inhibit entry of cells into S phase. This protein may serve as a link between p53-dependent cell cycle checkpoint and DNA repair. Recombinant human GADD45A protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Properties

Data Sheet	Click for Datasheet
Catalog Number	TP02177
Size	100 µg
Host	E.coli
Accession
Molecular Weight	19.4 kDa (173aa)
AP_Mol_Weight
Tag	N-6His
Sequences	MTLEEFSAGEQKTERMDKVGDALEEVLSKALSQRTITVGVYEAAKLLNVDPDNVVLCLLAADEDDDRDVALQIHFTLIQAFCCENDINILRVSNPGRLAELLLLETDAGPAASEGAEQPPDLHCVLVTNPHSSQWKDPALSQLICFCRESRYMDQWVPVINLPERLEHHHHHH
Purity	> 95% by HPLC
Concentration	0.5 mg/ml (determined by Bradford assay)
Formulation	Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol0.1M NaCl
Other Names	Growth arrest and DNA damage-inducible protein GADD45 alpha, DDIT1, GADD45
Bioactivity
Storage	Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Postscript	For research use only, not for use in diagnostic procedures.

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GADD45A, 1-165aa, Human, His tag, E.coli

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