ZCCHC17, also known as pNO40 or PS1D, is a 241 amino acid protein that interacts with both Pinin and the 60S ribosomal subunit. Localizing to nucleolus, ZCCHC17 is ubiquitously expressed and has been suggested to play a role in ribosome maturation and biogenesis. Recombinant human ZCCHC17 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.