WWOX, also known as WW domain containing oxidoreductase, is a proapoptotic protein and a tumor suppressor protein. WWOX is found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This protein may function synergistically with TP53/p53 to control genotoxic stress-induced cell death. It also plays a role in tumor necrosis factor (TNF)-mediated cell death. Recombinant human WWOX protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.