VTI1B, also known as v-SNARE, mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Recombinant human VTI1B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.