VAPA is a type IV membrane protein, present in the plasma membrane, intracellular vesicles and may also be associated with the cytoskeleton. This protein is a SNARE regulator with high levels of expression in the intestine during late embryogenesis and early neonatal development. VAPA may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Recombinant VAPA protein was expressed in E.coli and purified by using conventional chromatography techniques.