Vehicle-associated membrane (VAMP8) ,also known as endobrevin, is the main components of a SNARE complex involved in the docking and fusion of synaptic vesicles with the presynaptic membrane. This protein plays a role in regulated enzyme secretion in pancreatic acinar cells and is involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. It is required for dense-granule secretion in platelets. Recombinant VAMP8 protein was expressed in E.coli and purified by using conventional chromatography techniques.