ULBP2, also known as NKG2D ligand 2, is a member of a family of cell-surface proteins that function as ligands for human NKG2D. It could activate NKT cells and might indicate a mechanism by which clarithromycin improves the clearance of P. aeruginosa in chronic respiratory diseases. It is a useful serum biomarker for pancreatic cancer detection. Recombinant human ULBP2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.