UDP glycosyltransferase 8, also known as UGT8, catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system. Recombinant human UGT8 protein, fused to His-tag at N-terminus, was expressed in E.coli.