Uchl1 also known as Ubiquitin carboxyl-terminal hydrolase isozyme L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. UCHL1 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. Recombinant mouse Uchl1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.