UBTD2, also known as DCUBP (dendritic cellderived ubiquitin-like protein), is a ubiquitin (Ub) domain-containing protein first identified from dendritic cells, and is implicated in ubiquitination pathway. Ubiquitin is the most well understood post-translation modifier, there is a growing family of ubiquitin-like proteins (UBLs) that modify cellular targets in a pathway. UbL proteins are involved in a variety of cellular processes, including DNA repair, protein sorting, apoptosis, protein degradation, cell division and autophagy. Recombinant human UBTD2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.