UBL3, also known as PNSC1, is a 117 amino acid membrane protein belonging to the ubiquitin-like family. This protein contains two potential N-glycosylation sites, a potential protein kinase C phosphorylation site and a potential C-terminal prenylation site. Ubiquitin-like proteins are not directly involved in protein degradation, but appear to have many mechanistic similarities with the ubiquitin pathway. Recombinant human UBL3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.