UBE2V2, also known as ubiquitin-conjugating enzyme E2 variant 2, constitutes a distinct subfamily within the E2 protein family. They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. This protein also shares homology with ubiquitin-conjugating enzyme E2 variant 1 and yeast MMS2 gene product. It may be involved in the differentiation of monocytes and enterocytes. Recombinant human UBE2V2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.