Thioredoxin-like 4A, also known as TXNL4A, belongs to the Dim protein family. TXNL4A is a 142 amino acid protein that plays an essential role in pre-mRNA splicing. Due to a failure to express early zygotic transcripts, deletion of the gene encoding TXNL4A in Schizosaccharomyces pombe leads to embryonal lethality during gastrulation. Localized to the nucleus, TXNL4A interacts with hnRNP F, hnRNP H2, Cas-L and PQBP-1 to effect gene expression. Recombinant human TXNL4A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.