TWF1 is a ubiquitous actin-monomer-binding protein that is composed of two ADF-homology domains. It forms a 1:1 complex with ADP-actin-monomers, inhibits nucleotide exchange on actin monomers and prevents assembly of the monomer into filaments. This protein composed of two ADF/cofilin-like (ADF-H) domains connected by a short linker region and followed by a C-terminal tail of 20 residues. The two ADF-H domains are 20% homologous to ADF/cofilin and to each other. Recombinant human TWF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.