TSSC4 (tumor suppressing subtransferable candidate 4), also known as tumor suppressing STF cDNA 4 protein, belongs to the TSSC4 family. This protein is one of several tumor-suppressing subtransferable fragments located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. It plays a role in malignancies and disease that involve this region as well as hematopoietic cell function This protein was widely expressed in nearly all adult tissues, TSSC4 is also found in fetal liver, lung, kidney and brain. Recombinant human TSSC4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.