Cdc42-interacting protein 4, also known as TRIP10, belongs to the F-BAR family of proteins. These proteins contain an N-terminal, alpha-helical, region that is hydrophobic and considered to be related to the Bin Amphiphysin Rvs (BAR) protein family. Expressed in a variety of tissues, including kidney, brain, liver, lung, heart and pancreas, TRIP10 is required for the Insulin-dependent translocation of Glut4 to the plasma membrane and is essential for the coordination of membrane tubulation with Actin cytoskeletal reorganization during endocytosis. Recombinant human TRIP10 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.