Tpt1 also known as Translationally-controlled tumor protein. TPT1 is regulated at the translational level. This protein is involved in calcium binding and microtubule stabilization. It is an immunoglobulin E dependent histamine-releasing factor produced by lymphocytes of atopic children and present in biologic fluids of allergic patients. Recombinant mouse Tpt1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.