TMOD3 belongs to the tropomodulin family. Regulation of the actin cytoskeleton by filament capping proteins is critical to myriad dynamic cellular functions. The ability of these proteins to bind both filaments as well as monomers is often central to their cellular functions. The ubiquitous pointed end capping protein TMOD3 acts as a negative regulator of cell migration, yet mechanisms behind its cellular functions are not understood. Recombinant human TMOD3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.