Tlr2, also known as toll-like receptor 2, is a member of the Toll-like receptor (TLR) family. It is typically thought to recognize bacterial components, it has been described to alter the induction of both innate and adaptive immunity to a number of viruses, including vaccinia virus (VACV). It may recognize virus preparations in vitro and have a minor role in preventing dissemination of VACV following systemic infection with large doses of virus. it is wholly disposable in both control of virus replication and induction of adaptive immunity following intradermal infection. Recombinant mouse Tlr2, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.