TLR2, as known as toll-like receptor 2, belongs to the toll-like receptor (TLR) family. Human TLR family includes the members that activate the innate immune response via an ability to recognize molecular structures found in a variety of microbial pathogens. Specially, this protein is highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. It functions as part of a heterodimeric complex with either TLR1 or TLR6, and possibly other co-receptors. Recombinant human TLR2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.