TFPI2, as known as tissue factor pathway inhibitor 2 isoform 1, is a kunitz-type serine proteinase inhibitor, which is produced and secreted into endothelial cell matrix (ECM) by endothelial cells, smooth muscle cells, fibroblasts, keratinocytes, and urothelium. Also, this protein has been shown to inhibit ECM proteases essential for angiogenesis and metastasis. Recombinant human TFPI2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.