Small VCP/p97-interacting protein, also known as SVIP, is involved in a variety of cellular processes, including membrane fusion and ubiquitin-dependent protein degradation. SVIP functions as an inhibitor of the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway. Overexpression of SVIP, on the other hand, increased the levels of p62 protein and enhanced starvation-activated autophagy as well as promoted sequestration of polyubiquitinated proteins and p62 in autophagosomes. Recombinant human SVIP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.