Sulfotransferase 1C4, also known as SULT1C4, belongs to the SULT subfamily, responsible for transferring a sulfo moiety from PAPS to phenol-containing compounds. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. SULT1C4 catalyzes the sulfonation of p-nitrophenol and N-hydroxy-2-acetylaminofluorene, but not dopamine. Recombinant human SULT1C4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.