Sulfotransferase family, cytosolic, 1B, member 1, also known as SULT1B1, is an enzyme that in humans is encoded by the SULT1B1 gene. SULT1B1 contains a binding site for the sulfate donor, 3-prime-phosphoadenosine 5-prime-phosphosulfate, and a cysteine residue conserved in the ST1 gene family of sulfotransferases. Sulfotransferases such as SULT1B1 catalyze the biotransformation of a large number of endogenous compounds such as neurotransmitters, steroids, bile acids, and thyroid hormones, as well as drugs and xenobiotics. Recombinant human SULT1B1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.