Syntaxin-2, also known as STX2, belongs to thesyntaxin/epimorphin family of proteins. Syntaxin family is cellular receptors for transport vesicles which participate in exocytosis in neutrophils. STX2 is a t-SNARE that localizes to the apical plasma membrane and intracellular vesicular structures. Also, STX2, along with SNAP-23, is required for regulated surfactant secretion. Recombinant human STX2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.