Syntaxin 12, also known as, belongs to the syntaxin family. Syntaxin family is cellular receptors for transport vesicles which participate in exocytosis in neutrophils. STX12 regulates protein transport between late endosomes and the trans-Golgi network and interacts with ABC1 (ATP-binding cassette transporter A1), a protein that facilitates cellular release of choline-phospholipids and cholesterol to apoA-I (apolipoprotein A-I). Recombinant human STX12 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.